The Mycotoxin Summer Academy (MSA) from 1 - 12 July 2013 offered two subsequent one-week training courses. The first week provided an overview on the significance and properties of mycotoxins and toxigenic fungi including their determination and characterization. The second week was dedicated to various aspects of liquid chromatography coupled to mass spectrometry (LC-MS) especially for multi-analyte approaches to determine mycotoxins.