Combined MoniQA FST - „Analysis of Polychlorinated Biphenyls in difficult food matrices“ and “Mycotoxin Analysis in dried fruits, nuts and cereals using HPLC techniques”
Location: TUBITAK Marmara research center, Food Institute - Gebze, Turkey
Analysis of Polychlorinated Biphenyls in difficult matrices
The workshop will cover the analyses of PCB’s. The PCB’s extracted from a selected sample matrix and will be analysed by GC with ECD and MS detectors. The PAM method will be used for the extraction and clean-up of the PCBs.
Each Participant will have a chance to perform an extraction, a chromatographic analysis of a sample and will make contribution for the calculation of validation parameters with his/her own results. Some of method validation parameters (repeatability and intra-reproducibility and recovery) and evaluation of the results will be carried out jointly with two groups.
Theoretical part:
- General information on PCBs Analysis
- Operating principles of GC detection techniques.
- Sample extraction and clean-up techniques for PCBs analysis.
- Method Validation in Chemical Analysis*
Laboratory Practice part:
- Case Studies for PCBs in selected food matrices (eggs )
- Extraction and clean-up of PCB’s from a egg matrix
- GC determination of PCBs using GC-ECD and GC-MS detection techniques
- Parameters for Method validation (Repeatability, intra-reproducibility, recovery, accuracy) for the analysis
- Laboratory practice- Evaluation of results and validation parameters
Mycotoxin Analysis in dried fruits, nuts and cereals using HPLC techniques
The workshop will focus on mycotoxins in related matrices; Aflatoxins in hazelnut, Deoxynivalenol (DON) and Zearalenone(ZEA) in cereals, Ochratoxins A in raisins. HPLC and immunoaffinity clean-up methods will be used during the workshop. Participants will be divided to 3 groups of 3 participants. Each of the group will work on all mentioned mycotoxins.
You will also have a chance to learn about some of method validation parameters and how to evaluate the validation results like repeatability and intra-reproducibility, recovery and accuracy. They will have chance to work practically and theoretically on method validation of mycotoxins. Each participant will work individually and at the end of the workshop they will have their own report of repeatability, reproducibility, recovery and accuracy results.
Theoretical part:
- General information of mycotoxin analysis techniques
- Principles of Instrumental (HPLC) analysis
- Principles of mycotoxin analysis by HPLC
- Aflatoxins in hazelnut,
- Deoxynivalenol (DON) and Zearalenone(ZEA) in cereals,
- Ochratoxins A in raisins.
- Method validation in chemical analysis*
- Measurement uncertainty in chemical analysis*
- Mycotoxins and food safety
- Trends in mycotoxin analysis
Laboratory Practice part:
Case Studies for selected mycotoxin analysis which are aflatoxins in hazelnut, Deoxynivalenol (DON) and Zearalenone(ZEA) in cereals, Ochratoxins A in raisins.
- Sample handling and extraction of mycotoxins from the selected matrices
- HPLC determination of mycotoxins
- limited validation of the methods for selected mycotoxins
- Determination of validation parameters (Repeatability, intra-reproducibility, recovery, accuracy) for the analysis
- Determination of uncertainty for the analysis
- Evaluation of results and validation parameters
* Joint lectures for all participants.
The two workshops will be held in parallel.
MoniQA partners: free
Associated partners: 150 Euros
External institutions: 390 Euro
Reduced fee for PhD students: 75 Euros
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