Location: Ankara, Turkey
Organiser: Hacettepe University and CNR-ISPA
The workshop offers a broad view on Fusarium mycotoxins in cereals and derived products. It covers updated overview on the risk assessment and EU regulations of mycotoxins, various methodologies of toxin analysis and method validation protocols of analysis by the experts of the mycotoxin field. Laboratory applications for the analysis of deoxynivalenol and zearalenone in maize and derived products will be performed using both immunoaffinity clean-up methods and fast analysis methods. Participants will deal with sample extraction, clean up and identification of mycotoxins in cereal matrix extracts using HPLC techniques as well as with analysis by rapid test kits such as ELISA and lateral flow device. An LC-MS/MS method for simultaneous multi-toxin analysis will also be evaluated as a new approach in mycotoxin analyses.
The practical part of the three day training is open for 16 participants, and the participants will do the experiments in 4 groups (4 people in each group). This workshop is designed especially for people who want to develop skills in mycotoxin analyses and to gain experience on the validation of analytical methods which might help in selecting the right method.
Theoretical part:
- Overview on major mycotoxins and relevant toxigenic fungi
- Risks, risk assessment and regulations for mycotoxins
- Overview of various methodologies for mycotoxin analysis
- Criteria and parameters for the acceptance of analytical methods for mycotoxins
- A short overview on the analyses of the Fusarium mycotoxin by LC-MS/MS
- Comprehensive solutions for mycotoxin testing
Practical part:
- Laboratory applications on deoxynivalenol and zearalenone analysis by HPLC
- Extraction of the toxins from naturally contaminated and spiked samples
- Cleaning up of the extracts by immunoaffinity columns
- HPLC-UV/DAD and HPLC/FLD analysis of the samples
- Laboratory applications on deoxynivalenol and zearalenone analysis by rapid techniques (by ELISA and lateral flow device techniques)
- Laboratory applications on multimycotoxin analysis by LC-MS/MS
- Evaluation of the results of the analyses and validation parameters
- Comparison of the results with method performance criteria reported in EU Regulation No. 401/2006
Agenda
Transport and Accomodation
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